Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Thermo Scientific Production Chemicals and Services DOE AND INGALLS
NC3959188 ELUTE BUFFER B 50MM TRIS HCL8
Crescent Chemical Co Inc TAE BUFFER (10X) MOL BIO GRADE
TAE buffer (10x) molecular biology grade 10 x concentrated aqueous solution.TAE Buffer is used for the electrophoresis of nucleic acids. TAE has a lower buffer capacity than TBE, however linear ds DNA tends to run faster in TAE than in TBE. Size - 10L Sto
![Alkali Scientific TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 10 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659893.jpg-250.jpg)
Alkali Scientific TRIS Hydrochloride [Tris(hydroxymethyl) aminomethane HCl], 10 Kg
TRIS Hydrochloride (Tris(hydroxymethyl) aminomethane HCl) is a high-purity biochemical reagent used in various laboratory applications. The 10kg package provides a large supply of this important buffer solution, widely used in molecular biology, biochemistry, and cell culture studies. Tris Hydrochloride is commonly used in buffer formulations for protein electrophoresis, PCR (Polymerase Chain Reaction), and enzyme activity assays. It stabilizes pH levels during reactions, providing a stable environment for proteins and enzymes. TRIS is also essential in the preparation of DNA and RNA extraction buffers. This large-size packaging ensures an economical supply for high-throughput laboratories and research facilities.
![Alkali Scientific BIS TRIS Propane [1,3 Bis (TRIS(hydroxymethyl)methylamino)propane], 5 Kilograms](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659959.jpg-250.jpg)
Alkali Scientific BIS TRIS Propane [1,3 Bis (TRIS(hydroxymethyl)methylamino)propane], 5 Kilograms
BIS TRIS Propane (1,3 Bis (TRIS(hydroxymethyl)methylamino)propane) is a high-stability biochemical reagent, available in a 5-kilogram volume. It is used primarily as a pH buffer in high-performance applications that require precise pH control, particularly in biochemical research and protein studies. BIS TRIS Propane is known for its ability to maintain stable pH levels even in conditions where other buffers may fail. This reagent is often used in applications such as electrophoresis, protein purification, and enzyme assays. Its stability and low-temperature properties make it particularly useful in protocols that demand extended incubation periods or low pH variations.
![Alkali Scientific bis TRIS [bis (2 hydroxyethyl)aminotris(hydroxymethyl)methane], 100 G](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659960.jpg-250.jpg)
Alkali Scientific bis TRIS [bis (2 hydroxyethyl)aminotris(hydroxymethyl)methane], 100 G
Bis TRIS (bis(2-hydroxyethyl)aminotris(hydroxymethyl)methane) is a high-purity biological buffer available in 100g powder form. This buffer is widely used in molecular biology, biochemistry, and pharmaceutical research for applications requiring stable pH control in the alkaline range. Bis TRIS is especially effective in protein electrophoresis, enzyme assays, and cell culture environments where precise buffering is crucial. The 100g size is suitable for smaller laboratories or specific research applications. The buffer provides excellent stability, reducing the risk of pH fluctuations during sensitive experiments, and is frequently used in gel preparation, membrane protein studies, and DNA analysis workflows.
Alkali Scientific TRIS Buffered Saline, 10X Solution, 10 L
TRIS Buffered Saline (TBS) 10X solution is an essential reagent for biological and biochemical research. This 10L bottle provides a bulk supply of this concentrated buffer, ideal for large-scale laboratory use. TBS is widely used in immunology, protein chemistry, and molecular biology for procedures such as washing cells, diluting samples, and preparing buffers for antibody-based assays. The TRIS component ensures that the pH of the solution remains stable, which is critical for maintaining the integrity of proteins and other biomolecules. This 10X solution is easily diluted to 1X working concentration, making it versatile for a wide range of applications, including western blotting, ELISA, and PCR.
Alkali Scientific TRIS Buffered Saline, 10X Solution, 4 L
TRIS Buffered Saline (TBS) 10X solution is a concentrated buffer commonly used in molecular biology, immunology, and biochemistry. This 4L bottle provides a convenient, large-volume supply of buffer for high-throughput laboratories and research facilities. TBS is widely used in protocols such as washing cells, diluting proteins, and preparing buffers for antibody conjugation in applications like western blotting and ELISA. The TRIS component ensures that the pH remains stable, preventing fluctuations that could affect the accuracy of experimental results. This 10X solution is easily diluted to 1X, making it ideal for a variety of laboratory applications where stable pH and ionic strength are essential.
Alkali Scientific Tris Acetate Ultra Pure, 1 Kg
Tris Acetate Ultra Pure is a high-purity buffering agent used in a variety of biochemical applications. This reagent is ideal for high-precision applications such as electrophoresis, protein analysis, and other laboratory techniques requiring a stable pH. The ultra-pure grade ensures minimal interference with sensitive experiments, maintaining the integrity of reactions. This 1kg bottle is perfect for large-scale buffer preparations, providing consistent buffering power across a range of experiments. Tris acetate is commonly used in molecular biology, immunology, and protein chemistry, particularly for its ability to maintain a stable pH in high-purity systems.
Bioworld Tricine-SDS Sample Buffer, Non-Reducing (2X), 50 mL
Product DescriptionTricine-SDS is a sample buffer under non-reducing conditions for protein analysis. It has a concentration of 2X and contains 900 mM Tris-HCl with a pH of 8.45, 8% SDS, 20% glycerol, 0.01% Coomassie blue, and 0.01% phenol red.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.0) - 1L
Tris-HCl Buffer (1 M pH 8 0) - 1L (For Research Use and Further Manufacturing Only)