Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles500ML
Dulbeccos Modified Eagles500ML
Bio Rad Laboratories 1X PHOSPHATE BUFFERED SALINE W
Blocking Reagent; 1X Phosphate Buffered Saline With 1% Casein; Bio-Rad; 10mM sodium phosphate, 150um NaCl, 1% (w/v) casein, pH 7.4; 1L
Electron Microscopy Sciences D-PBS, Formula III Phosphate Buffer Saline 500 ML
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
Quality Biological Inc PBS (1X), pH 7.4 without Calcium and Magnesium, Case 10 X 500 mL
PBS (phosphate buffered saline) Balanced salt solution used for a variety biological and cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Also useful for immunological applications, such as Western Blotting.
Endotoxin and Mycoplasma tested
Without Calcium and Magnesium
Storage Condition: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
Endotoxin and Mycoplasma Tested
pH: 7.3 7.5
Osmolality: 300 ± 5% mOsm/Kg H2O
Max Shelf Life: 24 months from date of manufacture
Manufactured under an ISO 9001:2015 certified quality management system.
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.
Electron Microscopy Sciences Mops, Biological Buffer, 6.5-7.9 pH 100 GR
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged. Desired pH should be specified when ordering.
pH: 6.5-7.9
Teknova 1M Tris-HCl, pH 7.4. 1000 mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Sigma Aldrich Fine Chemicals Biosciences Dulbecco's Phosphate Buffered Saline Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 100ML
Dulbecco's Phosphate Buffered Saline Modified, without calcium chloride and magnesium chloride, liquid, sterile-filtered, suitable for cell culture | MFCD00131855 | 100ML
STEMCELL Technologies RoboSep™ Buffer 2, Size: 250 mL
RoboSep™ Buffer 2 is recommended for select EasySep™ cell separation protocols. Please note that one or two bottles of buffer are included with every purchase of a RoboSep™ Reagent Kit.
Sigma Aldrich Fine Chemicals Biosciences BICINE | 150-25-4 | MFCD00004295 | 25g
BICINE | Purity: ≥99% (titration) | MW:163.17 | 150-25-4 | MFCD00004295 | 25g
Boston Bioproducts Inc PIPES Buffer (1 M, pH 6.8) - 500ml
PIPES Buffer (1 M pH 6 8) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.6) - 1L
Tris-HCl Buffer (1 M pH 7 6) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris Buffered Saline, TBS (10X, pH 7.6) - 1L
Tris Buffered Saline TBS (10X pH 7 6) - 1L (For Research Use and Further Manufacturing Only)